Table 1. Real-time RT-PCR primer sequences.
| Gene | Forward sequence | Reverse sequence |
|---|---|---|
| GAPDH | CATGGCCTTCCGTGTTCCTA | GCGGCACGTCAGATCCA |
| Pax7 | AAATCCGGGACCGGCTGCTGAA | AGACGGTTCCCTTTGTCGCCCA |
| MyoD | GGATGGTGTCCCTGGTTCTTCAC | CTATGTCCTTTCTTTGGGGCTGGA |
| myogenin | AACTACCTTCCTGTCCACCTTCA | GTCCCCAGTCCCTTTTCTTCCA |
| VEGF-A | AGTGGCTTACCCTTCCTCATCTT | CGGGTCCTGCCCCATT |
| FGF2 | TGGTATGTGGCACTGAAACGA | TCCAGGTCCCGTTTTGGAT |
| BDNF | TAGCAAAAAGAGAATTGGCTG | TTTCAGGTCATGGATATGTCC |
| PGC1α | GGACAGTCTCCCCGTGGAT | TCCATCTGTCAGTGCATCAAATG |
| Neuronal NOS | GGTCTTCGGGTGTCGACAA | GAGTAGGCAGTGTACAGCTCTCTGA |
| Inducible NOS | GGATCTTCCCAGGCAACCA | CAATCCACAACTCGCTCCAA |
| Endothelial NOS | TTGTCTGCGGCGATGTCA | GAATTCTCTGCACGGTTTGCA |
| MHCe | GAGCAGCTGGCGCTGAA | TCTGATCCGTGTCTCCAGTTTCT |
| myostatin | ACCACGGAAACAATCATTACCAT | TGCCATCCGCTTGCATT |
| TNFα | ATGGCCTCCCTCTCATCAGT | CTTGGTGGTTTGCTACGACG |
| Atrogin1 | ACCGGCTACTGTGGAAGAGA | CCTTCCAGGAGAGAATGTGG |
| ATG5 | TTGAATATGAAGGCACACCCC | CTCTTGAAATGTACTGTGATGTTCCAA |
GAPDH; glyceraldehyde-3-phosphate dehydrogenase, Pax7; paired box transcription factor-7, MyoD; myogenic determination factor, VEGF-A; vascular endothelial growth factor-A, FGF2; fibroblast growth factor 2, BDNF; brain-derived neurotrophic factor, PGC1α; proliferator-activated receptor gamma coactivator 1-alpha, NOS; nitric oxide synthase, MHCe; myosin heavy chain embryonic, TNFα; tumor necrosis factor alpha, ATG5; autophagy-related gene 5