Fig 2. The FECPAKG2 standard operating protocol.
Three grams of fresh human stool is weighed in a Fill-FLOTAC device (step 1), water is added and the stool is homogenized using the Fill-FLOTAC device (Step 2 & 3). The fecal suspension is then transferred to a FECPAKG2 sedimenter (step 4) and water is added to the water line (step 5 & 6). The supernatant is removed by tilting the sedimenter towards the A-side (step 7) and floatation solution is added to the retained slurry until the saline line is reached (step 8). This mixture is transferred into a FECPAKG2 filtration unit by pouring from the B-side of the sedimenter (step 9). The content is mixed by inverting the filtration unit 3 times and a sample is extracted from the inside of the inner sieve using a pipette with special tip. Samples are pipetted into both wells of a FECPAKG2 cassette (step 10 & 11). The filled cassette is then placed on a level surface to allow accumulation of the helminth eggs (step 12). Finally, the cassette is placed in the MICRO-I device and both wells are imaged (step 13). Images, together with accompanying sample information is stored and uploaded to the cloud.