Table 1. Primers and probe used in this study.
| Primer (Position) | Sequence 5’→3’ | |
|---|---|---|
| SmLeish FWD Set 1 (2875–2895) | CCTCATCGCTTACCAGAATGT | |
| SmLeish Probe Set 1 (2897–2920) | TTACAAATCCACCCACCCACTCTGG | |
| SmLeish REV Set 1 (2948–2969) | TGGTTAGTATGCGCTCGAATTA | |
| SmLeish FWD Set 2 (749–770) | GTGATCTGAGGCACTATCTTCG | |
| SmLeish Probe Set 2 (790–817) | CAGTACTTTGAAGACCCGAGACTTGGTT | |
| SmLeish REV Set 2 (845–865) | CGGTAAATGGCACATAAGCTG | |
| SmLeish FWD Set 3 (1503–1525) | ATCCTATGCTTTCCTACGTGAAG | |
| SmLeish Probe Set 3 (1536–1561) | ACCACGTACACCTAGAGACCCTCAAA | |
| SmLeish REV Set 3 (1633–1652) | GCAGAAACCCAGATTCGTTG | |
| SmLeish expression *Fwd | CAC CAG GAG GAG GAG AGC CAT GGT ACC CTG TTC AAG | |
| SmLeish expression Rev | TTG TTT AAT TGA TCT ACG CCT G | |
*Forward primer modified by adding CACC nucleotides (italicized) and ribosome binding sequence (bold font) for efficient cloning and expression.