(a and b). Environmental O2 levels were manipulated using a Biospherix chamber. Differences in (a) bordering behavior and (b) roaming and dwelling behavior were suppressed in N2glb-5, npr-1 at lower environmental O2 levels. NS not significant, *p<0.05 by Wilcoxon-Mann-Whitney nonparametric test. (c). Fitness advantage of N2 over the barcoded N2glb-5, npr-1 strain was independent of environmental O2. NS, not significant, ***p<0.001 by ANOVA with Tukey’s Honest Significant Difference test. (d and e) Fitness differences of N2 and N2glb-5, npr-1 on (d) uniform bacterial lawns (UBL) where animals were unable to border, on plates containing agarose to prevent burrowing behaviors (NS, not significant, *p<0.05 by ANOVA with Tukey’s Honest Significant Difference test), and (e) on UV-killed bacteria (*p<0.05 by Wilcoxon-Mann-Whitney nonparametric test).
Figure 3—source data 1. Bordering rate at ambient (21%) and lower environmental (10%) O2 levels shown in Figure 3a.
Figure 3—source data 2. Roaming and dwelling behavioral assay in ambient (21%) and lower environmental (10%) O2 levels shown in Figure 3b.
Figure 3—source data 3. Fitness advantage of N2 over the barcoded N2glb-5, npr-1 strain was independent of environmental O2 shown in Figure 3c.
Figure 3—source data 4. Fitness differences of N2 and N2glb-5, npr-1 on uniform bacterial lawns (UBL) and on plates containing agarose shown in Figure 3d.
Figure 3—source data 5. Fitness differences of N2 and N2glb-5, npr-1 on UV-killed bacteria shown in Figure 3e.