Figure 3. Fitness advantage of N2 is independent of foraging behavior.
(a and b). Environmental O2 levels were manipulated using a Biospherix chamber. Differences in (a) bordering behavior and (b) roaming and dwelling behavior were suppressed in N2glb-5, npr-1 at lower environmental O2 levels. NS not significant, *p<0.05 by Wilcoxon-Mann-Whitney nonparametric test. (c). Fitness advantage of N2 over the barcoded N2glb-5, npr-1 strain was independent of environmental O2. NS, not significant, ***p<0.001 by ANOVA with Tukey’s Honest Significant Difference test. (d and e) Fitness differences of N2 and N2glb-5, npr-1 on (d) uniform bacterial lawns (UBL) where animals were unable to border, on plates containing agarose to prevent burrowing behaviors (NS, not significant, *p<0.05 by ANOVA with Tukey’s Honest Significant Difference test), and (e) on UV-killed bacteria (*p<0.05 by Wilcoxon-Mann-Whitney nonparametric test).