Figure 6. Feeding differences of strains containing derived alleles.

(a) N2 and N2_glb-5,npr-1 animals were synchronized by hatch-off and allowed to grow at the indicated O₂ levels for 72 hr. Video recordings were used to estimate the size of the animals. *p<0.05 by Wilcoxon-Mann-Whitney nonparametric test. (b) A previously published liquid, bacterial clearing assay was used to estimate food consumption for the N2_glb-5,npr-1 and N2 animals. On day 4, N2 animals had consumed more bacteria than N2_glb-5,npr-1animals. NS, not significant, ***p<0.001 by Wilcoxon-Mann-Whitney nonparametric test. (c). To test food consumption on agar plates, we developed a new assay by seeding 24-well agar plates with defined amounts of OP50-GFP bacteria. The number of bacteria on the plate could be estimated using a microplate reader. (d) N2 animals consumed more food than N2_glb-5,npr-1 regardless of foraging behaviors. ***p<0.001 by Wilcoxon-Mann-Whitney nonparametric test. (e) Schematic of CB4856 wild strain (blue) and a NIL (CX11400) containing the N2 allele of npr-1 from N2 (orange). (f) We tested the fitness effect of the N2 allele of npr-1 in the CB4856 wild strain using the CX11400 NIL strain. NS, not significant by Wilcoxon-Mann-Whitney nonparametric test. (g) Food consumption assays between CB4856 and N2 strains or CB4856 and the CX11400 NIL. ***p<0.001 by ANOVA with Tukey’s Honest Significant Difference test. (h) Pharyngeal pumping rates of N2, CB4856 and two NIL strains. *p<0.05, ***p<0.001 by ANOVA with Tukey’s Honest Significant Difference test.

Figure 6—figure supplement 1. Measurement of pharyngeal sizes of adult animals.

(a) Schematic of measurements made for the pharynx. (b) Pharyngeal sizes of adult animals, normalized to the N2 strain. ***p<0.001 by ANOVA with Tukey’s Honest Significant Difference test.