Figure 8. daf-22 is required for fitness differences of N2 and N2_glb-5,npr-1.

(a) Competition experiments between indicated strains. daf-22 encodes a sterol carrier protein, which is required for biosynthesis of most ascaroside pheromones. Red indicates the strain contain a deletion that spans the gene. **p<0.01 by Wilcoxon-Mann-Whitney nonparametric test. (b) Number of animals that carry fertilized eggs at the indicated timepoints. p=6.61×10⁻⁴ by Friedman test. (c) On plate feeding assays of the indicated strains. NS, not significant, ***p<0.001 by ANOVA with Tukey’s Honest Significant Difference test. (d and e) Attempts to rescue the relative food intake and reproductive timing defects of the daf-22 strain using crude pheromone. Neither of two concentrations of crude pheromone isolated from animals grown in liquid cultures had a significant effect on the two traits. NS, not significant, ***p<0.001 by ANOVA with Tukey’s Honest Significant Difference test. p=7.45×10⁻⁶ by Friedman test.