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. 2018 Oct 17;7:e38137. doi: 10.7554/eLife.38137

Figure 4. Cytonemes are essential for tissue-specific Bnl:GFP gradient profile.

(A-D) Representative images of Bnl:GFP uptake in CD4:mIFP-marked (red) clones in the ASP: (A) wt; (B) snRNAi, (C) diaRNAi; (D) nrgRNAi. (E) A box plot comparing the ratios of Bnl:GFP concentration outside (unmarked WT area) to inside (marked mutant area) of the CD4:mIFP-marked cytoneme-deficient mutant clones, expressing either diaRNAi (N = 3), nrgRNAi (N = 4), or snRNAi (N = 6) with that of the control marked WT clones (N = 3); P values, two-tailed t-test. (F,F’) Schematic models predicting differences expected in distribution patterns of EIF-stained Bnl:GFPex (red) on btl LOF clones (blue) either in diffusion-based (F’) or cytoneme-dependent (F) Bnl:GFP transport mechanism. Intracellular Bnl:GFP (green, Bnl:GFPin) level is expected to be reduced in both modes of signal transport. (G) Representative image showing autonomous loss of distribution of Bnl:GFPex (red, αGFP EIF-stained) in CD4:mIFP-marked (blue) btl LOF (btlRNAi) clone. (G’) Plot comparing relative Bnl:GFPex (red) density inside (mutant area) versus outside (WT area, control) of btl LOF clones (N = 3 independent ASPs); P values, two-tailed t-test. (H,H’) Representative image showing enrichment of αGFP EIF stained Bnl:GFPex (red) on long cytonemes (arrow) projected from btlGOF clones in the ASP stalk. Genotypes: hs-FLP/+; btl>y+>Gal4/+; UAS-CD4:mIFP, bnl:gfpendo/+ (A) or UAS-‘x’-RNAi (B–G) or UAS-btl (H’). Scale bars, 30 μm.

Figure 4—source data 1. Numerical data for measuring the ratio of Bnl:GFP concentration outside:inside of the marked clones in Figure 4E.
elife-38137-fig4-data1.xlsx (12.9KB, xlsx)
DOI: 10.7554/eLife.38137.027
Figure 4—source data 2. Numerical data for measuring Bnl:GFPex levels between btl LOF clones and the neighboring wt area in Figure 4—figure supplement 1G’.
elife-38137-fig4-data2.xlsx (11.4KB, xlsx)
DOI: 10.7554/eLife.38137.028
Figure 4—source data 3. Numerical data for measuring the relative Bnl:GFPex density in btl GOF clones and the neighboring wt area in Figure 4—figure supplement 1B.
elife-38137-fig4-data3.xlsx (9.5KB, xlsx)
DOI: 10.7554/eLife.38137.029

Figure 4.

Figure 4—figure supplement 1. Cytonemes are essential for Bnl:GFP gradient.

Figure 4—figure supplement 1.

(A) Table showing numerical data of clonal analyses for dia, nrg, and sn knockdown within the ASP. (B) Bar graphs comparing relative Bnl:GFPex (red) density inside versus outside (WT control, blue) of four different clones overexpressing Btl (btl GOF) in the ASP stalk region; each clone is from an independent biological sample.