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BLK was activated by KLF4 WT, not by KLF4 R458A. A. RNA-sequencing data showing that the BLK gene was only upregulated by KLF4 WT, but not by KLF4 R458A. B. Real time-PCR verified that BLK mRNA level was increased by ~2.2-fold in KLF4 WT expressing cells (P <0.05). C. Western blot analysis showed that BLK was upregulated ~3.9-fold by KLF4 WT (P <0.001), but was not changed by KLF4 R458A. Independent experiments were repeated at least three times.
