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PRDM5 protein expression was negatively regulated by DGCR5. (a) The protein level of PRDM5 in AGE1.HN and PC12 neurons was analyzed with western blot. (b) The expression of PRDM5 mRNA in AGE1.HN and PC12 cells was quantified by qRT-PCR. (c) After treated with CHX (12.5μg/mL) for several hours, the protein level of PRDM5 in AGE1.HN and PC12 cells was examined by western blot.
