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Selection of labelling positions in CaM and conformational changes upon peptide binding. Calmodulin adopts a dumbbell-like conformation and wraps around its target peptide as shown in the complexes with P1 and P2, respectively. Calmodulin is displayed in cartoon/surface representation with Phe92 colored in cyan and Val108 in orange. These positions display different degrees of solvent accessibility in the complexes and were exchanged for AzF for the IR measurements and crystallization.
