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Detection of serum autoantibodies against the p200 protein and type VII collagen by Western blotting with extract of human dermis. Sera from a patient with anti-p200/laminin γ1 pemphigoid and epidermolysis bullosa acquisita (EBA), respectively, showed IgG4 reactivity against the p200 antigen (lower arrow) and type VII collagen (upper arrow). In the right lane, a normal human serum (NHS) was applied.
