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. 2018 Nov 2;5:296. doi: 10.3389/fmed.2018.00296

Figure 7.

Figure 7

Detection of IgG and IgA autoantibodies against LAD-1 by Western blotting with the concentrated conditioned medium of cultured human keratinocytes. Sera from patients with bullous pemphigoid (BP), mucous membrane pemphigoid (MMP), both without BP180 NC16A IgG by ELISA, showed IgG reactivity with the soluble ectodomain of BP180 (LAD-1; left, arrow). In serum of a patient with linear IgA disease, IgA antibodies against LAD-1 were present (right). NHS, normal human serum.