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. 2018 Nov 2;9:442. doi: 10.3389/fgene.2018.00442

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Copyright © 2018 Fernández, Gennaro, Pirozzi, Baldo, Forzano, Turolla, Faravelli, Gastaldo, Coviello, Grasso and Bagni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of FMR1 mRNA and FMRP expression in different tissues. (A–C) Quantitative RT-qPCR detecting FMR1 mRNA relative expression in the tissues tested, normalized with the housekeeping HPRT1 mRNA [n = 3–5, technical replicates, independent experiments for (B,C)]. (D–F) Quantification of FMRP levels compared to GAPDH levels [n = 2–6 technical replicates, independent experiments for (E,F)]. The error bars indicate the standard error. Lower panels, representative Western blots from the different cell types analyzed. ^∗ indicates a non-specific signal which has been subtracted in the quantification.