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. 2018 Nov 2;9:2488. doi: 10.3389/fimmu.2018.02488

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Copyright © 2018 Alves, Araujo, Bernardes, Mendes, Oliveira and Fonseca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine production by spleen cells culture of immunized mice. Spleen from animals of MPLA (n = 11); Sm29 MPLA (n = 14); Alum (n = 11), Sm29 Alum (n = 15) and Infected and treated (n = 11) groups were obtained. Spleen cells cultured with (black bars) or without (open bars) rSm29 (25 μg/mL) stimulation was assessed to determine cytokine production. The levels of TNF-a (A), IL-6 (B), IL-10 (C), IL-17 (D), and IFN-γ (E) were measured by the CBA Th1/Th2/Th17 Kit. Statistically significant differences are denoted on the graphics. Bars represents the mean + SD values of three independent experiments.