Fig. 3. DISC1 exon 8 and exon 12 mutations converge on differential expression of a limited number of genes by RNA sequencing.

a–f RNA was collected from day 21 iNs and used for RNA sequencing. n = 4 for WT^ex8, 4 for MUT^ex8, 3 for WT^ex12, 4 for MUT^ex12. a Volcano plot of RNA sequencing data of all wild type (WT^ex8 and WT^ex12) vs all mutant (MUT^ex8 and MUT^ex12). Statistics: two-stage linear step-up procedure of Benjamini, Krieger, and Yejutieli, with Q = 5%. Dotted line shows threshold of significance for Q = 5%. b–f RNAseq data for individual genes as shown, by genotype. g–k Day 21 iN culture RNA was collected and used for qRT-PCR for select genes as shown, normalized to GAPDH. n = 12 for WT^ex8, 12 for MUT^ex8, 12 for WT^ex12, 12 for MUT^ex12, from three independent differentiations. See also Supp. Table 3