Fig. 6.

Inhibition of NFkB pathway re-sensitizes MLS resistant-cells to trabectedin treatment. a Total cell lysates from 402-91 WT and 402-91 ET cells, upon trabectedin treatment for the indicated time, were evaluated by WB for expression of HMGA1, E2F1, phospho-, and total HER3, HRGß1, phospho-IKKa/b and total IKKa, phospho- and total p65, and Hsp70. b Total cell lysates derived from 402-91 ET cells siScr and siHMGA1 untreated or treated cells were analyzed by WB for the expression of phospho- and total HER3, phospho- and total P65 and Hsp70. c–d 402-91 ET cells were transiently transfected with empty vector or vector containing IKB cDNA, and treated for 48 h with trabectedin. Total cell lysates from controls and transfected cells were analyzed by WB for the expression of IKB, HMGA1, phospho- and total p65, HRGβ1, phospho- and total HER3, Hsp70 and PARP. Cell death was evaluated by Trypan blue exclusion from three independent experiments (P < 0.01). e Nucleic and cytoplasmic fractions of 402-91 ET cells were analyzed for the expression of IKB, total and phospho-p65, PCNA and LaminA