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. 2018 Jul 9;37(45):5997–6009. doi: 10.1038/s41388-018-0386-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — PIM2 phosphorylates HK2 at Thr473. Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies. a, b HEK293T cells were overexpressed the indicated HA-tagged HK2 and Flag-tagged PIM2 (WT or K61A) proteins. Immunoprecipitation with an anti-HA antibody was performed. c A putative PIM2 substrate motif was identified in HK2. d, e HEK293T cells were overexpressed the indicated HA-tagged HK2 (WT or T473A) and Flag-tagged PIM2 (WT or K61A) proteins. Immunoprecipitation with an anti-HA antibody was performed. f Purified GST-tagged HK2 was mixed with the indicated bacterially purified His-tagged PIM2 proteins. An in vitro kinase assay was performed