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. 2018 Jul 9;37(45):5997–6009. doi: 10.1038/s41388-018-0386-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — PIM2 expression is positively correlated with pThr473-HK2 in human breast cancer. a, c Representative histopathologic sections of human breast normal and cancer tissues were stained with PIM2 or pThr473-HK2 antibodies. Scale bars, 50 μm. b, d Semi-quantitative immunohistochemical analysis of human breast normal and cancer tissues for PIM2 or pThr473-HK2. The experiments were tested with paired t-test, ***p < 0.001. e Pearson correlative analysis of semi-quantitative staining scores for PIM2 and pThr473-HK2. The standard curve was drawn by linear regression of the correlation scores. Correlation is shown using r and significance was determined using a Spearman correlation, ***p < 0.001