Table 1.
Primers used for cloning and RT-qPCR.
| Category | Primer | Sequence (5′-3′) | Product size (bp) |
|---|---|---|---|
| Fragment | Forward | GGGACGATCCAGTCACGCTG | 2,190 |
| Reverse | GACACCAACGGCTGCGCAG | ||
| 5′RACE | Reverse1 | GAGTGAAGAACAGTGA | 684 |
| Reverse2 | GTCATTCCCTCCAAGGTTCAAAGAAGTC | ||
| 3′RACE | Forward | CGAAGACAGGCGATGGGTT | 1,119 |
| Full-length | Forward | AGAGAGAGAAAACTGCCAGCC | 3,077 |
| Reverse | TTTCTGTCTCGACGGTCCTT | ||
| RT-qPCR | Forward | TCACACGCTTTCTACGGCTT | 122 |
| Reverse | AGGCTTAGGTCCAGTACGGT | ||
| GAPDH | Forward | CCCCACTGGCCAAGGTCATCA | 154 |
| Reverse | GCTGGGATGATATTCTGGTGGGC |