FIGURE 2.

Cytostatic effects of single treatment with metformin, diclofenac, diflunisal and 2-DG on the proliferation of myeloid leukemic cell lines. To analyze effects on proliferation of (A,D,G,J) THP-1, (B,E,H,K) U937 and (C,F,I,L) KG-1, 3 × 10⁴ cells/0.2 mL medium were seeded in 96-well plates with indicated concentrations of metformin, diclofenac, diflunisal and 2-DG. ³H-thymidine was added after 2 h and ³H-thymidine incorporation was determined after 20–22 h. (A–K) data are shown as mean ± SEM (n ≥ 4), ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 significance was calculated by ANOVA and post hoc Holm-Sidak’s multiple comparisons test. L data are shown as mean (n = 2).