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. 2018 Nov 2;9:1258. doi: 10.3389/fphar.2018.01258

FIGURE 4.

FIGURE 4

Metabolic effects of treatment with metformin, diclofenac and diflunisal on primary blasts. AML blasts were seeded in 24-well plates at a concentration of 5 × 105 cells/mL medium with or without metformin, diclofenac and diflunisal and 2-DG in the indicated concentrations. (A–C) After 24 h, cumulative lactate levels in cell culture supernatants were determined. (D–F) Cells were seeded in the same density in 24-well dishes with integrated optical oxygen (OxoDish®) sensors in the bottom of each well. The SensorDish® Reader was placed in the incubator for 24 h and measurements were performed in 30–60 s intervals. A,D show single treatments; in B,E diflunisal was combined with 0.1 mM diclofenac and in C,F triple treatments of diflunisal, diclofenac and metformin are shown. (A–C) data are shown as mean ± SEM (n ≥ 3), ∗P < 0.05 and ∗∗P < 0.01; significance was calculated by ANOVA and post hoc Holm-Sidak’s multiple comparisons test. (D–F) data are shown as mean (n = 3).