FIGURE 7.

Impact of cytarabine in combination with diclofenac and diflunisal on proliferation and apoptosis in AML cells. To analyze effects on proliferation of (A) THP-1 or (B) primary blasts 3 × 10⁴ cells/0.2 mL medium were seeded in 96-well plates with indicated concentrations of cytarabine, diclofenac and diflunisal. ³H-thymidine was added after 24 h and ³H-thymidine incorporation determined after another 24 h. For analysis of apoptosis, 3–5 × 10⁵ cells/mL medium were treated with or without cytarabine as a (C–F) single drug or (G,H) triple combination of cytarabine with diflunisal and diclofenac for 48 h. Cells were stained with Annexin-V-FITC and 7-aminoactinomycin D (7-AAD) and analyzed by flow cytometry. (A,B) Data are shown as mean ± SEM (two independent experiments in triplicate for THP-1, one experiment in triplicate for AML blasts). (C–H) Apoptosis was analyzed in one experiment with either THP-1 or primary blasts.