Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 2;9:2567. doi: 10.3389/fimmu.2018.02567

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Madel, Ibáñez, Rouleau, Wakkach and Blin-Wakkach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Analysis of pure ≥3N cells significantly improves OCL-specific outcomes for mouse differentiated cells. (A) Flow cytometry analysis and (B) quantification of CD51 and calcitonin receptor (CTR) positive cells. Using the same gating strategy as for cell sorting, total (gray) and 1–2N (orange) cells were compared to ≥3N cells (blue). Individual negative controls of each population are indicated as dotted lines in respective single parameter histograms and percentage of positive events is indicated. Data are presented as mean ±SEM of at least 5 independent cell preparations, each from 1 mouse. (C) FACS contour plot analysis on ≥3N cells for CD51 and CTR by plotting the nuclei number (H33342-A) against CD51 and CTR respectively. (D) Immunofluorescence analysis for CD51 and CTR on differentiated mouse cells. Nuclei were stained with DAPI. Scale bar = 50 μm. ns, not significant; ****p < 0.0001.