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. 2018 Nov 8;8:16542. doi: 10.1038/s41598-018-34520-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maternal calcium status affects the hepatic and adipose histology in the offspring mice. The mouse experimental procedure was the same as in Fig. 2, and three samples for each tissue in each group were randomly selected for histological analysis. (A) Oil red O staining (×400) of the liver in the offspring; (C): hematoxylin/eosin (HE) staining (×200 for 21 days, and ×100 for adults) of the epididymal fat in the offspring. (E) Oil red O staining (×400) of the liver in calcium insufficient female offspring with calcium supplementation. Changes in adipocyte size and hepatic lipid droplet quantity in the offspring mice affected by maternal calcium status were shown in (B) (21 day); (D) (adults) and (F) (calcium insufficient female offspring with calcium supplementation). The Image-pro Plus (IPP) was used to quantitatively analyze the size of fat cells in adipose tissues and the amount of lipid droplets in hepatic tissues. *Compared to the NC group, P < 0.05; ^#Compared to the LC-LC group, P < 0.05.