Table 1.

Fresh and dry weights (g plant⁻¹) of the transgenic plants (T3) with over-expression of AtHKT1 gene and the non-transgenic control (CK) under various NaCl stresses. CK is the “CV87” non-transgenic tobacco cultivar.

NaCl concentration (mmol L−1)	Group	Root fresh weight	Root dry weight	Shoot fresh weight	Shoot dry weight
0	CK	0.96 ± 0.02 a	0.05 ± 0.00 b	3.51 ± 0.13 ab	0.33 ± 0.01 ab
	T3	1.00 ± 0.01 a	0.06 ± 0.00 a	3.62 ± 0.02 a	0.34 ± 0.00 a
50	CK	0.52 ± 0.01 c	0.03 ± 0.00 d	2.50 ± 0.04 c	0.28 ± 0.01 c
	T3	0.98 ± 0.01 a	0.05 ± 0.00 b	3.65 ± 0.05 a	0.33 ± 0.00 a
100	CK	0.19 ± 0.00 e	0.02 ± 0.00 e	1.92 ± 0.02 d	0.24 ± 0.00 d
	T3	0.65 ± 0.01 b	0.04 ± 0.00 c	3.40 ± 0.03 ab	0.31 ± 0.00 b
150	CK	0.00 ± 0.00 f	0.00 ± 0.00 f	1.33 ± 0.01 e	0.14 ± 0.00 fg
	T3	0.24 ± 0.01 d	0.03 ± 0.00 d	3.07 ± 0.04 b	0.27 ± 0.01 c
200	CK	0.00 ± 0.00 f	0.00 ± 0.00 f	1.34 ± 0.01 e	0.12 ± 0.00 fg
	T3	0.15 ± 0.01 e	0.02 ± 0.00 e	1.93 ± 0.30 d	0.21 ± 0.00 e
250	CK	0.00 ± 0.00 f	0.00 ± 0.00 f	1.32 ± 0.01 e	0.14 ± 0.00 fg
	T3	0.00 ± 0.00 f	0.00 ± 0.00 f	1.36 ± 0.00 e	0.14 ± 0.00 f
300	CK	0.00 ± 0.00 f	0.00 ± 0.00 f	1.15 ± 0.04 e	0.12 ± 0.00 g
	T3	0.00 ± 0.00 f	0.00 ± 0.00 f	1.08 ± 0.00 e	0.11 ± 0.00 g

T3 is the transgenic tobacco line with highest AtHKT1 gene over-expression. Tukey’s HSD test is used in analysis of significant differences (P < 0.05) in the NaCl-stress (n = 9, i.e., 3 runs × 3 replications).