Figure 3.

Structure and Interfaces of the Collided Di-ribosome

(A) Overview of the collided di-ribosome structure. The two primary interfaces between the 40S subunits of the stalled and collided ribosomes are indicated. Red and cyan balls indicate the approximate sites of ubiquitination on eS10 and uS10, respectively. The ubiquitinated residues are not modeled, so the most proximal visible residues are indicated.

(B) Close-up view of interface 1. Proteins eS1, uS11, eS26, and eS28 are from the stalled ribosome and make contact with uS4 and h16 of the 18S rRNA of the collided ribosome. The mRNA in the stalled ribosome channel is shown.

(C) Close-up of interface 2 showing the approximate sites of eS10 and uS10 ubiquitination as in (A). RACK1 is from the stalled ribosome, while uS3, eS10, and uS10 are from the collided ribosome.

See also Figures S3 and S4 and Table S1.