REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-ZNF598	Abcam	Cat. #ab80458; RRID: AB_2221273
Rabbit monoclonal anti-eS10	Abcam	Cat. #ab151550; RRID: AB_2714147
Rabbit polyclonal anti-uS5	Bethyl Labs	Cat. #A303-794A; RRID: AB_11218192
Rabbit monoclonal anti-uL2	Abcam	Cat. #ab169538; RRID: AB_2714187
Rabbit monoclonal anti-eS24	Abcam	Cat. #ab196652: RRID: AB_2714188
Mouse monoclonal anti-Flag	Sigma-Aldrich	Cat. #F3165; RRID: AB_259529
Rabbit polyclonal anti-GFP	Chakrabarti and Hegde, 2009	N/A
Rabbit polyclonal anti-RFP	Chakrabarti and Hegde, 2009	N/A
HRP conjugated mouse monoclonal anti-beta-Actin	Sigma-Aldrich	Cat. #A3854; RRID: AB_262011
HRP conjugated goat anti-rabbit	Jackson ImmunoResearch	Cat. #111-035-003 RRID: AB_2313567
HRP conjugated goat anti-mouse	Jackson ImmunoResearch	Cat. #115-035-003; RRID: AB_10015289
Chemicals, Peptides, and Recombinant Proteins
3xFlag peptide	Sigma-Aldrich	Cat. #F4799
D-Biotin	Sigma-Aldrich	Cat. #47868 CAS: 58-85-5
Anti-Flag M2 affinity resin	Sigma-Aldrich	Cat. #A2220
Ni-NTA agarose	QIAGEN	Cat. #30210
Strep-Tactin Sepharose High-Performance	GE Healthcare	Cat. #28-9355-99
S7 Micrococcal Nuclease	Roche	Cat. #10107921001
Complete EDTA-free protease inhibitor cocktail	Roche	Cat. #11873580001
Pactamycin	Gift from E. Steinbrecher, Pharmacia Corp.	N/A
Cycloheximide	Sigma-Aldrich	Cat. #C4859; CAS: 66-81-9
Hygromycin B	Millipore	Cat. #400051-100KU CAS: 31282-04-9
Blasticidin S	Santa Cruz Biotechnology	Cat. #sc204655 CAS: 3513-03-9
Didemnin B	Shao et al., 2016	N/A
Emetine	Calbiochem	Cat #324693; CAS #316-42-7
Anisomycin	Sigma-Aldrich	Cat. #A9789; CAS #22862-76-6
Doxycycline	Sigma-Aldrich	Cat. #D9891; CAS: 24390-14-5
EasyTag L-[35S]-Methionine	Perkin Elmer	Cat. #NEG709A005MC
CAP (diguanosine triphosphate cap)	New England Biolabs	Cat. #S1404L
RNasin	Promega	Cat. #N251
Amino acid kit	Sigma-Aldrich	Cat. #09416
SP6 Polymerase	New England Biolabs	Cat. #M0207L
Creatine phosphate	Roche	Cat. #621714
Creatine kinase	Roche	Cat. #127566
His-Ubiquitin	Boston Biochem	Cat. # U-530
HA-Ubiquitin	Boston Biochem	Cat. # U-110
ZNF598-TEV-3xFlag (human)	Juszkiewicz and Hegde, 2017	N/A
eRF1-AAQ (human)	Brown et al., 2015a	N/A
UbcH5a	Boston Biochem	Cat. # E2-616
GST-UBE1 (human)	Boston Biochem	Cat. # E-306
Experimental Models: Cell Lines
HEK293T	ATCC	CRL-3216
ZNF598 KO Flp-In T-REx 293 dox inducible GFP-P2A-(KAAA)21-P2A-RFP	Juszkiewicz and Hegde, 2017	N/A
WT Flp-In T-REx 293 dox inducible GFP-P2A-(KAAA)21-P2A-RFP	Juszkiewicz and Hegde, 2017	N/A
WT Flp-In T-REx 293 dox inducible GFP-P2A-(K)0-P2A-RFP	Juszkiewicz and Hegde, 2017	N/A
E. coli BL21(DE3) pLysS	Thermo Fisher Scientific	C606003
Recombinant DNA
pSP64-Twin-Strep-VHPβ-K(AAA)20-3F4	This study	N/A
pcDNA3.1 ZNF598-TEV-3xFlag	Juszkiewicz and Hegde, 2017	N/A
pRSETA 6xHIS-TEV-eRF1(AAQ)	Brown et al., 2015a	N/A
Primer: SP64 5′ Fwd: TCATACACATACGATTTAGG	Sharma et al., 2010	N/A
Primer: SP64 Rev: CAATACGCAAACCGCCTC	Sharma et al., 2010	N/A
Primer: SP64 STOP-truncation Rev: CTATGACATG ATTACGAATTCCTATCCGGTTTTGAG CCCAGG	This study	N/A
Software and Algorithms
FlowJo	FlowJo	https://www.flowjo.com/
GraphPad Prism	GraphPad Software	https://graphpad.com/
RELION 2	MRC-LMB	https://www2.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page
UCSF Chimera	UCSF	https://www.cgl.ucsf.edu/chimera/
Pymol	Schrödinger	https://pymol.org/2/
Coot	MRC-LMB	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Adobe Illustrator	Adobe	https://www.adobe.com/uk/creativecloud.html
Other
SuperSignal West Pico Chemiluminescent substrate	Thermo Fisher Scientific	Cat. #34080
Rabbit Reticulocyte Lysate Mix	Sharma et al., 2010	N/A
DMEM, high glucose, GlutaMAX Supplement, pyruvate	Thermo Fisher	Cat. #10569010
Tetracycline-free Fetal Calf Serum (FCS)	BioSera	Cat. #FB-1001T/500
PonceauS Solution	Sigma-Aldrich	Cat. P-7170-1L; CAS: 6226-79-5
Qubit RNA HS Assay Kit	Thermo Fisher	Cat. #Q32852