REAGENT OR RESOURCE	SOURCE	IDENTIFIER
Antibodies
IC, immunocytochemistry; WB, western blot
Rabbit anti-G6Pase-β (IC)	Santa Cruz Biotechnology, Dallas, USA	Cat#sc-134714; RRID:AB_10647643
Rabbit anti-G6PT (WB)	Santa Cruz Biotechnology	Cat#sc-135479; RRID:AB_2254868
Donkey anti-mouse IgG-HRP (WB)	Santa Cruz Biotechnology	Cat#sc-2314; RRID:AB_641170
Donkey anti-rabbit IgG-HRP (WB)	Santa Cruz Biotechnology	Cat#sc-2077; RRID:AB_631745
Goat anti-GFAP (IC)	AbCam, Cambridge, UK	Cat#ab 53554; RRID:AB_880202
Rabbit anti-G6Pase-α (WB)	AbCam	Cat#ab 83690; RRID:AB_1860503
Rabbit anti-G6Pase-β (WB)	AbCam	Cat#ab 133964
Donkey anti-rabbit AlexaFluor 488 (IC)	ThermoFisher, Paisley, UK	Cat#A-21206; RRID:AB_141708
Donkey anti-goat AlexaFluor 633 (IC)	ThermoFisher	Cat#A-21082; RRID:AB_141493
Bacteria and Virus Strains
Lentiviral transfer vector, FUGW	Addgene	Addgene#14883
Envelope vector, pMD2.G	Addgene	Addgene#12259
Packaging vector, pMDLg/pRRE	Addgene	Addgene#12251
Packaging vector, pRSV.Rev,	Addgene	Addgene#12253
Packaging vector, psPAX2	Addgene	Addgene#12260
Chemicals, Peptides, and Recombinant Proteins
ATP	Sigma-Aldrich, Gillingham, UK	Cat#A9187
2-deoxy-d-glucose	Sigma-Aldrich	Cat#D6134
AlexaFluor 568-conjugated isolectin GS-B4	ThermoFisher	Cat#121412
Astrocyte growth medium (AGM BulletKit)	Lonza, Slough, UK	Cat#CC 3186
BAPTA	Molekula, Dorset, UK	Cat#20358510
Bovine serum albumin (BSA)	Europa Bioproducts Ltd, Cambridge, UK	Cat#EQBAH64
Cal-520AM	AAT Stratech Scientific, Suffolk, UK	Cat#21130
Dimethyl sulfoxide (DMSO)	Sigma-Aldrich	Cat#D2650
ECL Prime reagents	GE Healthcare Life Sciences, Little Chalfont, UK	Cat#RPN2232
ER Tracker Red	ThermoFisher	Cat#E34250
Fibronectin	Merck Millipore, Watford, UK	Cat#FC010
Fetal bovine serum (FBS)	Sigma-Aldrich	Cat#F7524, batch 094M3341
d-glucose-6-phosphate (G6P)	Sigma-Aldrich	Cat#G7879
Ionomycin	Apollo Scientific, Bredbury, UK	Cat#56092-81-0
Lipofectamine LTX	ThermoFisher	Cat#15338100
PAR-1 peptide, TFLLR	Tocris, Bristol, UK	Cat#1464
Probenecid	Sigma-Aldrich	Cat#P8761
Triton X-100	Sigma-Aldrich	Cat#T8787
Critical Commercial Assays
ATP assay kit	AbCam	Cat#ab113849
Glucose Uptake-Glo assay kit	Promega Southampton, UK	Cat#J1341
Fastlane cell cDNA kit	QIAGEN	Cat#215011
Experimental Models: Cell Lines
293FT cells	ThermoFisher	Cat#R70007
Human astrocytes from fetal cortex	Lonza, Slough, UK	Cat#CC-2565
Recombinant DNA
Plasmid (pEF/myc/ER FLIPglu-600uDelta13V) encoding ERglc600	Addgene	Addgene#18020
Plasmid (pEF/myc/ER FLIPglu-30uDelta13V) encoding ERglc30	Addgene	Addgene#18021
CCSB-Broad LentiORF-G6Pase-α	Dharmacon, Lafayette, USA	Cat#ccsbBroad304_04582
TRC human G6Pase-β shRNA	Dharmacon	Cat#TRCN0000051500
Quantitect primer assay: GAPDH	QIAGEN, Crawley, West Sussex, UK	Cat#Hs_GAPDH_1_SG
Quantitect primer assay: G6Pase-β	QIAGEN	Cat#Hs_G6PC3_1_SG
Quantitect primer assay: G6Pase-α	QIAGEN	Cat#Hs_G6PC_1_SG
Quantitect primer assay: GPBB	QIAGEN	Cat#Hs_PYGB_1_SG
Quantitect primer assay: GPMM	QIAGEN	Cat#Hs_PYGM_1_SG
Quantitect primer assay: GFAP	QIAGEN	Cat#Hs_GFAP_1_SG
Software and Algorithms
Prism 5	GraphPad, La Jolla, USA	https://www.graphpad.com/