Fig. 1.

GSK3B in medial prefrontal cortex is highly responsive to acute ethanol. a GSK3B protein in C57BL/6J mouse mPFC is phosphorylated at its inhibitory Serine-9 residue following i.p. injections of 0.5, 2.0, and 4.0 g/kg ethanol. Tissue for protein analyses was collected 30 min after injection. Representative Western blots of p-GSK3B-S9 and total GSK3B protein shown at top. Quantified intensity values (n = 5–6/dose) shown at bottom, with treatment effect significant at p < 0.001 by one-way ANOVA. Tukey’s post hoc testing revealed each dose differed significantly from saline control: **p < 0.01, ***p < 0.001. b Transcriptional response of Gsk3b RNA shows dynamic and robust response 4 h after injection of ethanol (1.8 g/kg). Direction of expression change varies across BXD panel, indicating genetic regulation of genomic response. Gsk3b 3’UTR transcript (probeset 1437001_at) shown in black, with exonic Actb (probeset 1436722_a_at) shown for reference (grey)