Fig. 3.

CA1-specific Nampt knockdown recapitulates cognitive hypersensitivity observed in old mice. a Nampt mRNA levels in the CA1, CA3, and DG regions of CA1-specific Nampt knockdown and GFP-injected control mice (n = 3 mice for each group; *p < 0.05, unpaired Student’s t-test). b Total ambulations were quantified during the 1 h locomotor activity test conducted on CA1-specific Nampt knockdown mice (n = 8) and GFP-injected control mice (n = 7). c, d Analysis of the MWM probe trial data did not reveal any significant differences between CA1-specific Nampt knockdown (n = 8) and GFP-injected control (n = 7) mice (unpaired Student's t-test, two-way rmANOVA). e–g Contextual fear conditioning tests were conducted on CA1-specific Nampt knockdown (n = 8) and GFP-injected control (n = 7) male mice. Values of percent time freezing are shown from the tone-shock (CS-US) training period on day 1 (e), contextual fear conditioning on day 2 (f), and the auditory cue period on day 3 (g). Statistical analyses were conducted by two-way rmANOVA, and significant group effects are denoted by a symbol (§) at the right sides of the graphs; *p < 0.05, **p < 0.01, ***p < 0.001. h Shock sensitivities for flinch response, vocalization, and escape response are shown for the same groups of mice used in (e–g) (*p < 0.05, unpaired Student’s t-test). Data are presented as mean ± SE