Fig. 8.

Differential clustering of 4-1BB receptors on Jurkat cells induced by urelumab and utomilumab. a Confocal images of 4-1BB receptor co-clusters with Urelumab-Alexa Fluor 488 conjugate (URE, green), Urelumab-Fab Alexa Fluor 488 Conjugate (URE-Fab), Utomilumab Alexa Fluor 594 conjugate (UTO, red), or with a combination of URE and UTO in the presence (top row) or absence (bottom row) of 4-1BB His-Avi ligand. Nuclei are stained with DAPI (blue). Scale bar = 10 µm. b Numbers of observed clusters per 100 µm² of cellular area. Data shown as mean + S.E.M., N = at least 10 cells from three independent experiments. *p-value ≤ 0.05; **p-value ≤ 0.01; ***p-value ≤ 0.001; n.s., not significant