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. 2018 Nov 8;8:16544. doi: 10.1038/s41598-018-35037-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Recovery of Treg cells after treatment with 0.5 µg of DT for two consecutive days. DEREG mice (n = 4–5) were treated for two consecutive days with 0.5 µg of DT and the frequency of Treg cells in the blood was followed in the next 7 days. The frequency of CD4⁺Foxp3⁺ cells was measured by flow cytometry. As can be seen, Treg cells returned to normal frequency at the day 7 of treatment (A,B). Treg cells reached the lowest frequency at day 2 after treatment and progressive recovery their level in the next 5 days (B). The experiment was repeated 3 times with similar results.