Fig. 5.

Thymic VEC proliferation is developmentally coordinated with ETP prevalence. a Total thymic cells from postnatal day 2 (P2) (N = 7, two experiments) and 4-week-old mice (4 W) (N = 6, two experiments) (cellularity from 1 lobe). Bars show mean ± s.e.m. b Percentage of phenotypically defined DN1 thymic progenitor cells (Dapi-Lineage-CD4-CD8-CD25-c-Kit+) from postnatal day 2 (P2) (N = 7, two experiments) and 4-week-old (4 W) mice (N = 6, two experiments). Bars show mean ± s.e.m. P-value is shown (Student’s t-test). c Percentage of phenotypically defined DN2 thymic progenitor cells (Dapi-Lineage-CD4-CD8-CD25+c-Kit+) from postnatal day 2 (P2) (N = 7, two experiments) and 4 weeks old (4 W) mice (N = 6, two experiments). Bars show mean ± s.e.m. P-value is shown (Student’s t-test). d DN1 and DN2 thymic progenitor cells from postnatal day 2 (P2) and 4-week-old mice (4 W). Plots are representative of two experiments. e Percentage of phenotypically defined Ki67+VECs (CD45-Ter119-EpCAM-CD31+) from postnatal day 2 (P2) and 4-week-old mice (4 W). Bars show mean ± s.e.m. f Ki67 expression in perinatal (P2) and 4-week-old mice (4 W) thymic VECs. Plots are representative of two experiments