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. 2018 Nov 8;8:16589. doi: 10.1038/s41598-018-34405-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Embryonic development of the surface-dwelling form of Asellus aquaticus. (A) An embryo at ~6 days post fertilization. (B) At ~10 days post fertilization, the head (H) and the tail (T) are distinct. Yolk is (Y). (C) Lateral view of embryo at ~12 days post fertilization with the chorion (C) starting to shed off. (D) Lateral view of embryo now extending while membrane (C) continues to be shed. (E) Lateral view of embryo with defined head (H) and tail (T) and three membranes remaining. (F). At ~26 days, the second membrane is shed, the yolk size is reduced, and eye and head pigmentation (E) is obvious. (G) Lateral view after second membrane is shed and there is an increase in eye (E) and head pigmentation. (H) At ~32 days, the embryo sheds the third membrane and the eye and head have increased pigmentation.

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