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. 2018 Nov 8;8:16589. doi: 10.1038/s41598-018-34405-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Eye region of surface and cave forms of Asellus aquaticus. (A–C) Surface embryos from RS population at 75% of the way through embryonic development (A), 85% of the way through embryonic development (B) and 95% of the way through embryonic development (C). By the end of embryogenesis (C), embryos have 3 pigmented spots (ommatidia are difficult to see because of pigmentation). (D–F) Surface embryos from a light colored variant from the PP surface population develop small circular fragments (E) which then combine into ommatidia (F). Fragments and ommatidia shown within the red circles. (G–I) Cave embryos at the same timepoints show no forming ommatidia. Anterior is to the left.

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