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. 2018 Nov 8;9:4695. doi: 10.1038/s41467-018-07163-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Src-phosphorylated p120-catenin recruits Anillin to the junctions. a The effect of constitutively active and dominant negative RhoA on vSrc-driven extrusion. Embryos were injected with the following constructs: dUAS:EGFP-vSrc, dUAS:EGFP-vSrc;CA-RhoA or dUAS:EGFP-vSrc;DN-RhoA. The two graphs display cells Apically extruded (outside of the embryo) and Tall (within the monolayer but taller than neighbours). Data are mean ± s.d. of 3 independent experiments (total number of embryos: n_Src = 35; n_Src,CA-RhoA = 38; n_Src,DN-RhoA = 36). *P < 0.05 (Student’s t-test). b A schematic model of the domain composition of p120-catenin. In green are phosphorylation sites regulated by the Src kinase (from: PhosphoSitePlus database). In the bottom panel, a design of p120-catenin mutant with two sites regulating the interaction between p120-catenin and RhoA⁴⁴. These sites are mutated from Y to F (p120-mutFF). c The effect of phosphomimetic p120-mutFF on vSrc-driven extrusion. Embryos were injected with the following constructs: dUAS:EGFP-vSrc;p120-wt or dUAS:EGFP-vSrc;p120-mutFF. Data are mean ± s.d. of three independent experiments (total number of embryos: n_Src,p120-wt = 34; n_{Src,p120-mutFF} = 42). *P < 0.05 (Student’s t-test). d, e Time-lapse imaging of the effect of phosphomimetic p120-mutEE on the localisation of Anillin-GFP in cells arrested at the G2/M transition. Embryos were injected with a combination of the following constructs: dUAS:Cherry-Wee1;CA-Cdc25 and dUAS:p120-mutEE;Anillin-GFP. Movies were taken over 4 h. Frames were extracted from a representative movie at indicated times from the tailbud stage in xy (d) and xz (e) view. Scale bars, 40 µm (d) and 20 µm (e). f Time-lapse imaging of Anillin-GFP localisation in cells arrested at the G2/M transition. Embryos were injected with a combination of the following constructs: dUAS:Cherry-Wee1;CA-Cdc25 and dUAS:myr-Cherry;Anillin-GFP. Movies were taken over 4 h. Frames were extracted from a representative movie at indicated times from the tailbud stage. Scale bars, 50 µm. g Time-lapse imaging of the effect of p120-mutFF on the localisation of Anillin-GFP in cells arrested at the G2/M transition. Embryos were injected with a combination of the following constructs: dUAS:Cherry-Wee1;CA-Cdc25 and dUAS:p120-mutFF;Anillin-GFP. Movies were taken over 4 h. Frames were extracted from a representative movie at indicated times from the tailbud stage. Scale bars, 25 µm