Fig. 8.

vSrc modulates apical polarity complex to facilitate extrusion. a A schematic model of regulation of apicobasal polarity by Cdc42 (adapted from ref.⁴⁹). The Par3-Par6-aPKC complex is recruited to the cell surface in a Par3-dependent manner⁴⁶, and is regulated by apically localised Cdc42. Active Cdc42 stimulates phosphorylation, dissociation and relocation of Par3 to the apical-lateral border at tight junctions (TJ), while Par6-aPKC moves apically to define the apical domain^46,47,49. b The effect of dominant negative aPKC on vSrc-driven extrusion. Embryos were injected with the following constructs: dUAS:EGFP-vSrc and dUAS:EGFP-vSrc;DN-aPKC. Data are mean ± s.d. of three independent experiments (total number of embryos: n_Src = 31; n_Src,DN-aPKC = 31). *P < 0.05 (Student’s t-test). c The effect of dominant negative Par3 on vSrc-driven extrusion. Embryos were injected with the following constructs: dUAS:EGFP-vSrc and dUAS:EGFP-vSrc;DN-Par3. Data are mean ± s.d. of three independent experiments (total number of embryos: n_Src = 36; n_Src,DN-Par3 = 34). *P < 0.01 (Student’s t-test). d The effect of overexpression of WASP-RBD-GFP on vSrc-driven extrusion. Embryos were injected with the following constructs: dUAS:EGFP-vSrc and dUAS:EGFP-vSrc;WASP-RBD-GFP. Data are mean ± s.d. of three independent experiments (total number of embryos: n_Src = 31; n_{Src,WASP-RBD-GFP} = 36). *P < 0.01 (Student’s t-test). e The effect of phosphomimetic aPKC-Y417F on vSrc-driven extrusion. Embryos were injected with the following constructs: dUAS:EGFP-vSrc;aPKC-wt or dUAS:EGFP-vSrc;aPKC-Y417F. Data are mean ± s.d. of three independent experiments (total number of embryos: n_Src,aPKC-wt = 39; n_{Src,aPKC-Y417F} = 42). *P < 0.05 (Student’s t-test)