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. 2018 Nov 8;8:16549. doi: 10.1038/s41598-018-34812-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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L. donovani maintains high levels of synteny with L. major including chromosome 22 where the A2 genes are located. Left: Dot plot of the coding DNA sequences of L. major compared to those of L. donovani generated from our assembly across the entire genome. Right: Synteny comparison of chromosome 22. The outer most circle represents the chromosomal location. The second circle is labelled with genes on the forward strand (blue) and genes on the reverse strand (red). The third circle represents genes that are only present in one of the two compared species. The inner association lines join syntenic genes between the two species.