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. 2018 Nov 8;8:16549. doi: 10.1038/s41598-018-34812-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Summary of all genes with non-synonymous mutations between the cutaneous, visceral, and gain-of-function strains of L. donovani. All non-synonymous SNPs and Indels were classified as common to our previous study (2014 CL⁴) or identified in this study (Novel), as well as by their effect on amino acid changes from top to bottom, colored red to green in descending order of likelihood to affect the phenotype of the parasite. 66 genes were common to the previous data set. Of those genes, 7 were previously investigated⁴ and 1 was rejected due to an open reading frame misannotation. 25 genes were only listed in this study (Novel). Diagram created using SankeyMATIC (http://sankeymatic.com).