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. 2018 Nov 8;9:4694. doi: 10.1038/s41467-018-07149-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Actomyosin-enriched inner corset is under tension. a A schematic of half of the gonad showing where laser incisions were performed in b, e, f, and g. Green color represents actomyosin regulators enriched region while magenta color represents germ cell membrane. Asterisk shows the site of point incision and black lines shows the site of line incision along the distal to proximal axis, perpendicular line incision at the rachis surface, and ablation at the germ cell membrane. b Time-lapse images of the recoiling of germ cell membrane after laser incision at the rachis of control and nmy-2 (RNAi) germlines. An incision was made in the direction of the distal to proximal axis (yellow line ~9 µm). White double-headed arrow shows the displacement at indicated time point. c Displacement of the adjacent membranes over time after ablation in control (N = 24) versus nmy-2 (RNAi) (N = 19) germlines. d Quantification of initial recoil velocity after laser incision at the rachis in control (N = 24) versus nmy-2 (RNAi) (N = 19) germlines. e Time-lapse images of the recoiling of rachis surface after point ablation. Dotted circle represents isotropic displacement of the nearby surface over time. f Time-lapse images of the recoiling of rachis surface after a line incision made perpendicular to the direction of the distal to proximal axis (yellow line ~9 µm). White double-headed arrow shows displacement at indicated time points. g Time-lapse images of shape deformation of the rachis bridges after laser incision at the germ cell membranes (orange—rachis bridge away from the incision site; yellow—rachis bridge near to the incision site). h Displacement of the adjacent rachis surface over time after point (N = 15) and perpendicular line incision (N = 15). i Quantification of initial recoil velocity after point (N = 15) and perpendicular line incision (N = 14). j Change in the circularity of rachis bridge over time. Error bar denotes standard deviation (s.d.), data represents mean ± s.d. (*p < 0.05, ***p < 0.0001) of three independent experiments. Statistical analysis was done using Mann–Whitney U-test, N = number of gonads analyzed and scale bar represents 10 µm. Scale bar in the magnified images of (a) represents 5 µm