Figure 3.

For a Figure360 author presentation of Fig. 3, see the figure legend at https://doi.org/10.1016/j.bpj.2018.9.019#mmc3

Axial stretch at variable rates after colchicine and PTL treatment of RCMs. (A) A transmitted light image of isolated adult RCM before and during tensile test. (B) The VE model (a Maxwell element in parallel configuration with a Voigt element) for which the time dependence of the stress-strain curve can be used to extract values for each parameter. The top row shows the imposed length change—a 20 μm length controller step over either a 200 ms (C and E) or 5 s (D and F) stretch, followed by a 5 s hold and 200 ms return. The middle row shows the average change in SL in response to stretch. The bottom row shows the average change in force in response to stretch. (A and B) 100 μm/s stretch and 2 μm/s stretch for DMSO and colchicine (N = 5 hearts, n = 17 cells DMSO, n = 18 cells colchicine). (C and D) 100 μm/s stretch and 2 μm/s stretch for DMSO and PTL. (N = 5 hearts, n = 14 cells DMSO, n = 17 cells colchicine). Solid traces overlaid on force-relaxation curves are best fits to double exponential decay function. To see this figure in color, go online.