Figure 1.

Generation of Several Hybrid Exons by Genomic Edition Using Pairs of sgRNAs

The formation of hybrid exons was tested with various pairs of previously characterized sgRNAs (Table 2) that could, in principle, restore an open reading frame for the DMD gene. The formation of the hybrid exon was confirmed by PCR amplification, (i.e., there was no PCR product when the hybrid exon was not formed). As a negative control (Ct), we used non-transfected HEK293T cells.