Table 1.

List of sgRNA Target Sites in Exons 46 to 58

sgRNA No.	Exon No.	Strand	sgRNA Target Sequences, Excluding PAM (5′–3′)	sgRNA Target Sequence Position	PAM Nucleotide Position	Cut Sites in the DMD Gene	Cut Sites in the Amino Acid Sequence	sgRNA Used in the Formation of the Hybrid Exon
1	46	sense	TTCTCCAGGCTAGAAGAACAA	1407207–1407227	1407228–1407233	6624 – 6225	2208 GAA (Glu): 2209 CAA (Gln)	46–51; 46–53
2	46	antisense	CTGCTCTTTTCCAGGTTCAAG	1407312–1407332	1407306–1407311	6714 –6715	2238 CTT (Leu): 2239 GAA (Glu)	46–53
3	47	sense	GTCTGTTTCAGTTACTGGTGG	1409686–1409706	1409707–1409712	6769 –6770	2257 G:TG (Val)	47–58
4	47	antisense	TCCAGTTTCATTTAATTGTTT	1409736–1409756	1409730–1409735	6824 –6825	2268 AAA (Lys): 2267 CAA (Gln)	47–58
5	47	antisense	CTTATGGGAGCACTTACAAGC	1409765–1409785	1409759–1409764	6833 – 6834	2278 CT:T (Leu)	47–58
6	49	antisense	TTGCTTCATTACCTTCACTGG	1502716–1502736	1502710–1502715	7194 –7195	2398 CCA (Pro): 2399 GTG (Val)	49–52; 49–53
7	51	antisense	TTGTGTCACCAGAGTAACAGT	1565282–1565302	1565276–1564281	7323 –7324	2441 ACT (Thr): 2442 GTT (Val)	46–51
8	51	antisense	AGTAACCACAGGTTGTGTCAC	1565294–1565314	1565288-1565293	7335 –7336	2445 GTG (Val): 2446 ACA (Thr)	46–51
9	52	antisense	TTCAAATTTTGGGCAGCGGTA	1609765–1609785	1609759–1609764	7595 –7596	2532 AC:C (Thr)	47–52
10	52	sense	CAAGAGGCTAGAACAATCATT	1609802–1609822	1609823–1609828	7647 −7648	2549 ATC (Ile): 2550 ATT (Ile)	49–52
11	53	antisense	TTGTACTTCATCCCACTGATT	1659891–1659911	1659885–1659890	7677 – 7678	2559 AAT (Asn): 2560 CAG (Gln)	49–53
12	53	sense	CTTCAGAACCGGAGGCAACAG	1659918–1659938	1659939–1659944	7719 – 7720	2573 CAA (Gln): 2574 CAG (Gln)	46–53
13	53	sense	CAACAGTTGAATGAAATGTTA	1659933–1659953	1659954–1659959	7734 –7735	2578 ATG (Met): 2579 TTA(Leu)	46–53
14	53	sense	GCCAAGCTTGAGTCATGGAAG	1660017–1660037	1660038–1660043	7818 –7819	2606 TGG (Trp): 2607 AAG (Lys)	46–53
15	53	antisense	CTTGGTTTCTGTGATTTTCTT	1660068–1660088	1660062–1660067	7854 – 7855	2618 AAG (Lys): 2619 AAA (Lys)	46–53
16	58	sense	TCATTTCACAGGCCTTCAAGA	1860349–1860369	1860370–1860375	8554 –8555	2852 A:AG (Lys)	47–58
17	58	antisense	CAGAAATATTCGTACAGTCTC	1860411–1860431	1860405–1860410	8601 –8602	2867 GAG (Gln): 2868 ACT (Thr)	47–58
18	58	antisense	CAATTACCTCTGGGCTCCTGG	1860467–1860487	1860461–1860466	8657 – 8658	2886 GT:C (Gln)	47–58

Nucleotide positions are provided with reference to the DMD gene sequence ENS00000198947 analyzed using the Benchling web tool (https://benchling.com/). Bold underlined sequences are intron sequences.