Table 2.

Analysis of Hybrid Exon Amplicons by TIDE

Upstream sgRNA	Downstream sgRNA	Expected Size of the Amplicon (bp)	Correct Junction (%)
1-46	7-51	967	90.8
1-46	8-51	955	86.1
1-46	12-53	1,099	54.6
1-46	13-53	1,084	60.9
2-46	14-53	1,090	N/A
2-46	15-53	1,054	N/A
5-47	9-52	997	N/A
6-49	10-52	1,386	83.3
6-49	11-53	1,548	84.7
3-47	16-58	587	90.7
4-47	17-58	575	N/A
5-47	18-58	548	67,7