Table 2.
Upstream sgRNA | Downstream sgRNA | Expected Size of the Amplicon (bp) | Correct Junction (%) |
---|---|---|---|
1-46 | 7-51 | 967 | 90.8 |
1-46 | 8-51 | 955 | 86.1 |
1-46 | 12-53 | 1,099 | 54.6 |
1-46 | 13-53 | 1,084 | 60.9 |
2-46 | 14-53 | 1,090 | N/A |
2-46 | 15-53 | 1,054 | N/A |
5-47 | 9-52 | 997 | N/A |
6-49 | 10-52 | 1,386 | 83.3 |
6-49 | 11-53 | 1,548 | 84.7 |
3-47 | 16-58 | 587 | 90.7 |
4-47 | 17-58 | 575 | N/A |
5-47 | 18-58 | 548 | 67,7 |