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Off-Target Analysis of the Corrected and Control Human Heterozygous Embryos
Genomic DNA from seven corrected and three control human embryos was collected, and the genomic DNA was extracted and amplified by PCR. The PCR products were analyzed by deep sequencing. The percentage of C-to-T within a 10-nt window near the PAM-distal end of the protospacer at 32 potential off-target sites was calculated. The PAM sequences are underlined; the mismatch sites are highlighted in red.
