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. 2018 Oct 2;115(9):1755–1761. doi: 10.1016/j.bpj.2018.09.024

Figure 5.

Figure 5

Diverse rhomboid proteases are dimeric in detergent micelles (A) A representative single-molecule TIRF microscopy image of GlpG-HaloC-His in DDM detergent micelles that was captured onto a coverslip coated with NiNTA. (B) Quantification of photobleaching step events of the indicated rhomboid proteins that were captured onto coverslips coated with NiNTA in the indicated detergent micelles (labeled above each bar; NG is nonyl-glucoside). HiGlpG-NCys was directly labeled with maleimide-JF549, whereas the other proteins were labeled via their Halo tags. (C) Quantification of the photobleaching step events of the indicated control proteins as in (B). To see this figure in color, go online.