Figure 2.

Migratory and Proliferative Capacities of C-AdMSCs and D-AdMSCs

(A) Representative image of colony-forming unit assays stained with cresyl violet from C-AdMSCs and D-AdMSCs. (B) Graph depicting the number of AdMSC colonies. Note the decreased number of colonies in D-AdMSCs. (C) Graph depicting the size of the AdMSC colonies. Note the decreased colony size in D-AdMSCs. (D) Quantification of Ki67+ cells, revealing a reduction in the percentage of proliferative cells in D-AdMSCs. (E) Immunocytochemistry against Ki67 (green) in C-AdMSCs and D-AdMSCs. Cell nuclei were stained with Hoechst dye (blue). (F) Representative flow cytometric analysis of BrdU incorporation by C-AdMSCs and D-AdMSCs. Percentage of cells expressing BrdU is indicated. Cells were exposed to BrdU for 3 hr before cell fixation. (G) Graph depicting the percentage of reduction of the scratched area after 48 hr. (H) Representative phase contrast images of a scratch assay showing the migration of AdMSCs after 48 hr. (I) Graph depicting the number of migrated cells through a transwell membrane after 48 hr. (J) Cresyl violet staining of AdMSCs that transmigrated through a transwell membrane after 48 hr. Scale bars: 100 μm in (E and H) and 50 μm in (J). Data are represented as mean ± SEM. *p < 0.05, **p < 0.001 (two-tailed t test).