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. 2018 Nov 1;8(6):441–451. doi: 10.1007/s13659-018-0187-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Effect of COD on renal histopathological alterations in DKD rats. The photomicrographs of H&E-stained (a) and PAS-stained (c) kidney sections were obtained for the four groups on day 30. Scale bar: 600 μm (×100). b Histopathological alterations in H&E-stained kidney slides were scored for the four groups on the basis of the semi-quantitative percentage of the damaged area on a scale from 0 to 4: 0, normal; 1, changes < 25% of cortical area; 2, changes 25–50% of cortical area; 3, changes 50–75% of cortical area; 4, changes > 75% of cortical area. d Score results of the glomerular area of the four groups. Magnification: ×200. Glomerular area was presented as the mean ± SD. ^##p < 0.01, ^#p < 0.05 vs. Con group; *p < 0.05, **p < 0.01 vs. COD treatment (n = 6)