Fig. 4.

Myeloid cell-specific SIRT1 deletion increases pimonidazole uptake into M1 ATMs in epididymal adipose tissue of high-fat-fed mice. a, b Pimonidazole uptake into M1 (a) and M2 (b) ATMs. ATMs in epididymal adipose tissue of control mice (blue line) and Mye-SIRT1 KO mice (red line) fed a high-fat diet for 6 weeks were analyzed using flow cytometry. The representative results of four independent experiments are shown. c Staining with anti-F4/80 antibody (left panels) and immunostaining with anti-pimonidazole antibody (right panels) of epididymal fat tissues of mice fed a high-fat diet for 6 weeks. Scale bars indicate 100 μm. d Pimonidazole-positive area in epididymal adipose tissues of fl/fl (white bar) and KO mice (black bar). Data are shown as the mean ± SEM. *P < 0.01