Figure 4.

GCAWKR Correlates With and Regulates PTP4A1

(A) The correlation between GCAWKR transcript level and PTP4A1 mRNA level was measured in 35 GC tissues. The ΔCt values (normalized to β-actin) were subjected to Spearman rank-correlation analysis. (B) qRT-PCR and western blot assays were performed in SGC7901 and BGC823 cells after transfection of GCAWKR shRNAs. n = 3, nonparametric Mann-Whitney test. Error bars in the bar graphs represent SD. (C) The PTP4A1 expression was detected with real-time PCR and western blot in SGC7901 and BGC823 cells after transfection of lentivirus harboring the full-length human GCAWKR sequence or the empty vector. (D) Luciferase reporter vector was generated by inserting the promoter region (−2,000 to 0 bp) of the PTP4A1 gene. The reporter vectors were then cotransfected into SGC7901 and BGC823 cells with GCAWKR or control shRNAs. Cells were harvested for luciferase activity assay. Results shown are the mean ± SD of triplicate determination from three independent experiments. *p < 0.05.